For example, if the total energy is 2 billion calories, what is the total biomass?
How do you calculate the total biomass when only the total energy is given?
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No. Different molecules have different quantities of energy per mass. For instance fats are higher in energy per mass than carbohydrates. Therefore an organism that has a high proportion of fat will have more energy per mass than an organism that has a high proportion of carbohydrates. Therefore 2 billion calories of an organism that is high in fat will weigh less than 2 billion calories of an organism that is high in carbohydrates.